An Agrobacterium rhizogenes mediated hairy root transformation protocol for fenugreek
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چکیده
منابع مشابه
Agrobacterium Rhizogenes-mediated Transformation of Peganum multisectum (Maxim) Bobrov and Harmine Production in Hairy Roots
Using Agrobacterium rhizogenes due to create hairy roots is a useful method to product secondary metabolites in many medicinal plants. The transgenic hairy roots were induced from Peganum multisectum (Maxim) Bobrov a medicinally important species, by infecting leaf and stem explant with wild type Agrobacterium rhizogenes strain ATTCC 15834, which led to the induction of hairy roots from 19% of ...
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Gloriosa superba L. a medicinally important monocot, member of the family Liliaceae, was investigated for callusing, direct regeneration and induction of hairy root using soil bacterium Agrobacterium rhizogenes. Friable callus was initiated from corms on basal MS medium amended with 2,4-D (1 mg L) and kinetin (1 mg L). Callus generated from such effective combinations were co-cultivated with th...
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The Agrobacterium-mediated transformation of hairy roots creates a rapid and simple means to introduce and express foreign genes in plant cells that are capable of synthesizing specific natural products. We describe a method for the production of transgenic hairy root cultures of peanut (Arachis hypogaea L.) using Agrobacterium rhizogenes R1000, a strain with the binary vector pBI121. We evalua...
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ژورنال
عنوان ژورنال: MethodsX
سال: 2020
ISSN: 2215-0161
DOI: 10.1016/j.mex.2020.101098